DETERMINATION OF GLUCOSE-OXIDASE BY MEANS OF THE CHEMILUMINESCENCE OFCYPRIDINA LUCIFERIN ANALOG

Authors
MITANI M KOINUMA Y KOSUGI M
Citation
M. Mitani et al., DETERMINATION OF GLUCOSE-OXIDASE BY MEANS OF THE CHEMILUMINESCENCE OFCYPRIDINA LUCIFERIN ANALOG, Analytical sciences, 10(2), 1994, pp. 267-269
Citations number
8
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical sciences → ACNP
ISSN journal
09106340
Volume
10
Issue
2
Year of publication
1994
Pages
267 - 269
Database
ISI
SICI code
0910-6340(1994)10:2<267:DOGBMO>2.0.ZU;2-S
Abstract
FeCl2 was found to be a useful catalyst for the determination of H2O2 based on the chemiluminescence of a Cypridina luciferin analogue, hoxy phenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA). By this method, H2O2 was detected in a range from 300 pmol/ml to 10 nmol/ml. In addit ion, glucose oxidase (GOD) was determined in a range from 1.18 x 10(-5 ) units/ml (280 amol/ml) to 7.56 x 10(-4) units/ml (17.9 fmol/ml) when GOD was incubated with glucose for 10 min at 34-degrees-C.