A HIGHLY SENSITIVE QUANTITATIVE-DETERMINATION OF GLUTATHIONE IN PLANT-ROOTS BY HIGH-PERFORMANCE GEL-FILTRATION CHROMATOGRAPHY AND FLUOROMETRIC DETECTION AFTER PRECOLUMN DERIVATIZATION WITH N-(7-DIMETHYLAMINO-4-METHYL COUMARINYL)-MALEIMIDE

Authors
OBATA H IMAI K INOUE N UMEBAYASHI M
Citation
H. Obata et al., A HIGHLY SENSITIVE QUANTITATIVE-DETERMINATION OF GLUTATHIONE IN PLANT-ROOTS BY HIGH-PERFORMANCE GEL-FILTRATION CHROMATOGRAPHY AND FLUOROMETRIC DETECTION AFTER PRECOLUMN DERIVATIZATION WITH N-(7-DIMETHYLAMINO-4-METHYL COUMARINYL)-MALEIMIDE, Phytochemical analysis, 5(5), 1994, pp. 239-242
Citations number
9
Categorie Soggetti
Biology,"Chemistry Analytical","Plant Sciences
Journal title
Phytochemical analysis → ACNP
ISSN journal
09580344
Volume
5
Issue
5
Year of publication
1994
Pages
239 - 242
Database
ISI
SICI code
0958-0344(1994)5:5<239:AHSQOG>2.0.ZU;2-V
Abstract
A convenient method was developed for the highly sensitive, quantitati ve determination of glutathione (GSH) using high performance gel filtr ation chromatography and fluorescence detection after N-(7-dimethylami no-4-methyl coumarinyl)-maleimide modification of the peptides. The fl uorescence detection method is highly sensitive and sub pmol GSH conte nts were detectable. This method is applicable for determining the GSH content of micro samples from higher plant roots.