QUANTITATIVE-ANALYSIS OF ALIZARIN IN TISSUE-CULTURES OF RUBIA SPECIESBY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
LODHI AH SANTANA AEG CHARLWOOD BV
Citation
Ah. Lodhi et al., QUANTITATIVE-ANALYSIS OF ALIZARIN IN TISSUE-CULTURES OF RUBIA SPECIESBY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Phytochemical analysis, 5(5), 1994, pp. 261-265
Citations number
19
Categorie Soggetti
Biology,"Chemistry Analytical","Plant Sciences
Journal title
Phytochemical analysis → ACNP
ISSN journal
09580344
Volume
5
Issue
5
Year of publication
1994
Pages
261 - 265
Database
ISI
SICI code
0958-0344(1994)5:5<261:QOAITO>2.0.ZU;2-6
Abstract
A rapid, high performance liquid chromatographic (HPLC) method has bee n developed for the quantitative analysis of alizarin in field grown r oots and tissue cultures of Rubia tinctorum and R. peregrina. Alizarin was quantified by HPLC in the presence of trichlorophenol as internal standard using an RP C-18 Nova-Pak(R) column eluted isocratically wit h methanol: 10% aqueous acetic acid (60:40). The minimum detection lim it of alizarin was found to be 10.5 ng. The field grown roots and call us of R. tinctorum contained, respectively, 939.6 (+/- 17.4) and 335.2 (+/- 4.9) mug/g dry weight total alizarin, whilst similar tissues of R. peregrina contained 147.9 (+/- 10.0) and 216.8 (+/- 0.4) mug/g dry weight total alizarin.