ANTIBODIES TO THE T-CELL RECEPTOR (CD3) INDUCE LFA-1 MAC-1-DEPENDENT MONONUCLEAR CELL-ADHESION TO HUMAN ARTERIAL ENDOTHELIUM

Monoclonal antibodies to the CD3 determinant on T lymphocytes have bee n used extensively as immunosuppressive agents. The T-cell receptor fo rms a complex with CD3 so that antibodies to CD3 mimic T-cell receptor cross-linking by human leukocyte antigens. Both CD3 and T-cell recept or cross-linking have been associated with lymphocyte activation and e xpression of the LFA-1 adhesion ligand, which leads to adhesion to int ercellular adhesion molecule 1 immobilized on artificial surfaces. We sought to determine if anti-CD3 antibodies would increase adhesion of heart transplant recipient peripheral blood mononuclear cells to confl uent human aortic endothelial cells by a similar mechanism. Peripheral blood mononuclear cells were incubated for 30 minutes with monoclonal CD3 or CD3 + CD18 antibody, before a 15-minute incubation with unstim ulated human aortic endothelial cells. Mononuclear cell adhesion doubl ed after anti-CD3 antibody preincubation from 15.1 +/- 2.1 to 29.3 +/- 6.7 cells/high-power field (p = 0.002). This increase was abolished b y coincubation with anti-CD18 (7.1 +/- 3 cells/high-power field; p = 0 .0002). The major increase was the result of increased lymphocyte adhe sion (3.4 +/- 0.6 to 14.8 +/- 6.7 cells/high-power field; p = 0.02); h owever, monocyte adhesion also increased from 11.7 +/- 1.5 to 14.5 +/- 1.6 cells/high-power field (p = 0.04). Anti-CD18 antibodies markedly reduced binding of both cell types. Cross-linking of the T-cell recept or by antibodies to CD3 causes acute adhesion of mononuclear cells (pa rticularly lymphocytes) to arterial endothelium. Increased adhesion is mediated through CD18 (LFA-1/Mac-1).