5-AMIDO-(CARBOXYFLUORESCEIN)2'-DU-OLIGONUCLEOTIDES - NOVEL PRIMERS FOR FLUORESCENT DETECTION OF PCR AMPLIFIED DNA

Authors
JADHAV VR BARAWKAR DA NATU AA GANESH KN
Citation
Vr. Jadhav et al., 5-AMIDO-(CARBOXYFLUORESCEIN)2'-DU-OLIGONUCLEOTIDES - NOVEL PRIMERS FOR FLUORESCENT DETECTION OF PCR AMPLIFIED DNA, Nucleosides & nucleotides, 16(1-2), 1997, pp. 107-114
Citations number
24
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
1-2
Year of publication
1997
Pages
107 - 114
Database
ISI
SICI code
0732-8311(1997)16:1-2<107:5-NPF>2.0.ZU;2-V
Abstract
The synthesis of oligonucleotides containing a novel nucleobase fluore scent derivative 5-NH2-(carboxyfluorescein)-2'-deoxyuridin in which th e chromophore fluorescein is directly linked to nucleobase of 2'dU at C-5 via an amido function is described. The fluorescent oligonucleotid es have been used as primers for PCR amplification of Human beta-globi n gene fragment. Oligonucleotides containing multiple biotechnology.