RAPID DIAGNOSIS OF APPLE PROLIFERATION MYCOPLASMA-LIKE ORGANISM USINGA POLYMERASE CHAIN-REACTION PROCEDURE

Authors
FIRRAO G GOBBI E LOCCI R
Citation
G. Firrao et al., RAPID DIAGNOSIS OF APPLE PROLIFERATION MYCOPLASMA-LIKE ORGANISM USINGA POLYMERASE CHAIN-REACTION PROCEDURE, Plant Pathology, 43(4), 1994, pp. 669-674
Citations number
19
Categorie Soggetti
Plant Sciences",Agriculture
Journal title
Plant Pathology → ACNP
ISSN journal
00320862
Volume
43
Issue
4
Year of publication
1994
Pages
669 - 674
Database
ISI
SICI code
0032-0862(1994)43:4<669:RDOAPM>2.0.ZU;2-C
Abstract
The rapid diagnosis of apple proliferation mycoplasma-like organism, u sing polymerase chain reaction amplification of part of the 16S rRNA g ene is reported. Apple proliferation MLO can be detected in as little as 0.3 g of diseased tissue. Results compare favourably with those obt ained by other PCR-based methods. Operational speed, sensitivity, spec ificity and ease of performance of the method, with particular referen ce to indexing purposes, are discussed.