THE IDENTIFICATION OF A PATHOTYPE-SPECIFIC DNA-PROBE FOR THE R-TYPE OF PSEUDOCERCOSPORELLA-HERPOTRICHOIDES

Authors
NICHOLSON P REZANOOR HN HOLLINS TW
Citation
P. Nicholson et al., THE IDENTIFICATION OF A PATHOTYPE-SPECIFIC DNA-PROBE FOR THE R-TYPE OF PSEUDOCERCOSPORELLA-HERPOTRICHOIDES, Plant Pathology, 43(4), 1994, pp. 694-700
Citations number
28
Categorie Soggetti
Plant Sciences",Agriculture
Journal title
Plant Pathology → ACNP
ISSN journal
00320862
Volume
43
Issue
4
Year of publication
1994
Pages
694 - 700
Database
ISI
SICI code
0032-0862(1994)43:4<694:TIOAPD>2.0.ZU;2-I
Abstract
A 6.7-kb DNA fragment has been isolated from an R-type isolate of Pseu docercosporella herpotrichoides that exhibits specific hybridization t o R-type isolates and not to W, C or S pathotypes or to P. anguioides. R-type isolates are polymorphic with respect to this fragment and thr ee sizes were present (11.6, 6.7 and 4.5 kb) in EcoRI-digested DNA fro m isolates of a world-wide collection. Infection of rye seedlings was demonstrated by hybridization of this probe to DNA extracted from infe cted plants.