CHANGES IN PROTEIN SPECTRA OF TRANSGENIC PLANTS CARRYING DIFFERENT AGROBACTERIUM-TUMEFACIENS C58 T-DNA GENES

A series of binary vector plasmids derived from the T-DNA of the Agrob acterium tumefaciens strain C58, carrying the five plant morphoregulat ory genes 1, 2, 4, 5 and 6b in different combinations, was used in the transformation of Nicotiana tabacum leaf discs. Protein patterns of t he transgenic tobacco analysed through SDS-PAGE have shown changes in the polypeptides with M(r): approximately 120, 60, 55, 43 and 27 kDa ( for tobacco with transgene 4); approximately 60, 55, 43, 26-25, 21, 18 kDa (for tobacco with transgenes 1, 2 and 5); approximately 70, 60, 2 6, 25, 18 kDa (for tobacco with transgene 5); approximately 60, 55, 48 , 26, 18 kDa (for tobacco with transgenes 4, 5, 6b); approximately 60, 55, 22 and 18 kDa (for tobacco with transgene 6b); approximately 60, 55, 43, 26 and 18 kDa (for transgenes 5, 6b); approximately 60, 55, 22 , 18 and 16 kDa (for transgenes 4 and 6b). All types of transgenic pla nts showed quantitative changes in protein content. Mendelian segregat ion ratio to kanamycin resistance in the progeny of transgenic tobacco clones in the R1 generation was 3:1 except in transgenic tobacco carr ying transgenes 1, 2 and 5.