GLYCOSYLATED ALPHA-CHYMOTRYPSIN IN REVERS ED MICELLES OF AEROSOL-OT IN OCTANE AS A MODEL IN STUDYING THE ROLE OF CARBOHYDRATE FRAGMENTS IN GLYCOPROTEINS

Authors
RARIY RV KLYACHKO NL MARTINEK K LEVASHOV AV
Citation
Rv. Rariy et al., GLYCOSYLATED ALPHA-CHYMOTRYPSIN IN REVERS ED MICELLES OF AEROSOL-OT IN OCTANE AS A MODEL IN STUDYING THE ROLE OF CARBOHYDRATE FRAGMENTS IN GLYCOPROTEINS, Bioorganiceskaa himia, 20(3), 1994, pp. 249-256
Citations number
12
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
20
Issue
3
Year of publication
1994
Pages
249 - 256
Database
ISI
SICI code
0132-3423(1994)20:3<249:GAIREM>2.0.ZU;2-L
Abstract
A comparative study of the well known non-glycosylated enzyme alpha-ch ymotrypsin, and its derivative artificially glycosylated with D-glucos amine was carried out to reveale the effect of carbohydrate fragments on oligomeric composition and membrane activity of glycoproteins. A mi croheterogeneous system of reversed micelles of Aerosol OT in octane, which can be a model of the biomembrane environment of enzymes in vivo , was used as a medium for enzymatic reactions. It was found that the glycosylation of alpha-chymotrypsin makes the protein to be enables to oligomerization and interaction with the micellar matrix (membrane af finity).