MONOCLONAL-ANTIBODIES AGAINST TRYPSIN-BIN DING DOMAIN OF HUMAN URINARY TRYPSIN-INHIBITOR

Three MAb M2, B6, P1 (IgG1 type) against human urinary trypsin inhibit or (UTI), a glycoprotein with antiinflammatory properties, have been p roduced by hybridization of mouse mueloma cells P3O1 with spleen cells of immunized mice BALB/c. Competitive ELISA-examination of the peroxi dase conjugates of M2, B6, and P1 MAb in the presence of the trypsin b inding domain shows the M2 antibody to possess the highest affinity fo r this domain. On the basis of the MAb M2, a competitive ELISA of UTI concentration in urine is proposed. ELISA-detectable changes in the UT I content of urine from patients with nephritis without renal failure can be considered as an early index of renal parenchyma damage.