IN-VITRO BULBLET REGENERATION FROM BULBSCALE EXPLANTS OF LILIUM-JAPONICUM THUNB - EFFECT OF PLANT-GROWTH REGULATORS AND CULTURE ENVIRONMENT

The interaction of auxin (NAA), cytokinins (BA, kinetin, 2iP and zeati n) and culture environment (light and temperature) on bulblet producti on from bulbscale explants of Lilium japonicum Thunb. were investigate d. A combination of NAA and 2iP, in general, gave a better response th an combinations involving other cytokinins. An incubation temperature of 20-degrees-C and continuous illumination were better than growing a t 25-degrees-C in the dark. Regenerated bulblets predominantly origina ted from the basal end of explants in the control medium and this pola rity was reversed by supplementing the medium with NAA + BA or NAA + 2 iP. The importance of an illuminated environment for leaf induction in regenerated bulblets is discussed.