IN-VIVO PRODUCTION OF HEAT-SHOCK PROTEIN IN MOUSE PERITONEAL-MACROPHAGES BY ADMINISTRATION OF LIPOPOLYSACCHARIDE

The in vivo production of heat shock protein was studied by administra tion of bacterial lipopolysaccharide (LPS) into mice. Heat shock prote in 70 was detected in the extract of adherent peritoneal cells from mi ce injected intraperitoneally with LPS by using the immunoblotting met hod. The expression of heat shock protein 70 was found 2 days after in jection of LPS and reached its peak 4 days after injection. The intrap eritoneal injection of LPS induced the expression of heat shock protei n 70, whereas its subcutaneous injection did not. The in vivo producti on of heat shock protein 70 was inhibited by administration of LPS tog ether with quercetin, an inhibitor of accumulation of heat shock prote in 70 mRNA. Tumor necrosis factor alpha enhanced LPS-induced heat shoc k protein production in vivo There was a decrease of gamma delta T cel ls in the peritoneal cavity of mice injected intraperitoneally with LP S. It was suggested that bacterial LPS is a stressful agent which indu ces the in vivo heat shock protein response, and its administration le ads to the production of heat shock protein 70 in peritoneal macrophag es.