THE COMPLETE NUCLEOTIDE-SEQUENCE OF COSMID VECTOR PTL5 - LOCATION ANDORIGIN OF ITS GENETIC COMPONENTS

The complete nucleotide sequence (5793 bp) of the cosmid Vector pTL5 a nd the origin of its genetic components has been determined. Cosmid pT L5, a derivative of cosmid vector pHC79, is composed of genetic compon ents from pBR322, bacteriophage lambda and the hybrid lambdoid bacteri ophage Charon (Ch) 4A cohesive ends (cos) region. The Ch4A cos region contains genetic components from two bacteriophages, the lambda cos-le ft arm and the phi 80 cos-right arm regions. The Ch4A cos region has b een used in the construction of many other cosmid-type vectors, some o f which have been sequenced and entered into the GenBank database.