S. Koby et al., THE CHITINASE ENCODING TN7-BASED CHIA GENE ENDOWS PSEUDOMONAS-FLUORESCENS WITH THE CAPACITY TO CONTROL PLANT-PATHOGENS IN SOIL, Gene, 147(1), 1994, pp. 81-83
A transposon system based on Tn7 carrying the chiA gene (encoding chit
inase) of Serratia marcescens was established. The transposon was intr
oduced by conjugation into Pseudomonas fluorescens as a stable integra
te expressing and secreting the active chitinase enzyme. The resulting
strain was shown to control the plant pathogen Rhizoctonia solani in
soil.