The activated sludge process is an aerobic biological method for organ
ic matter reduction from waste water. The objective of this research w
as to conduct a survey of micro-organisms present in 10 activated slud
ge plants. The following groups of organisms were investigated; eubact
eria, filamentous bacteria, fungi, yeasts, algae and protozoa. Eubacte
ria, fungi and yeasts were isolated on casitone glycerol yeast extract
agar, rose bengal chloramphenicol agar and yeast malt extract agar re
spectively. Twenty-two different genera of eubacteria were isolated an
d identified as mainly gram-positive rods belonging to the genus Bacil
lus. Spore-forming bacteria predominated over non-spore formers. Micro
thrix parvicella was the most common filamentous organism detected in
the activated sludge plants studied. When compared to the rest of the
microflora, fungi and yeasts were not detected in large magnitude, mos
tly belonging to common genera. Algal types detected were the common f
resh-water and polluted water algae. Protozoans were well represented
with the most common types being Paramecium and Euplotes spp.