IN-VITRO INTERACTIONS BETWEEN AMPHOTERICIN-B AND HALOACETAMIDE DERIVATIVES AGAINST CANDIDA SP

Antifungal activities of amphotericin B (AmB) and haloacetamide deriva tives were studied against 17 strains of Candida albicans and 6 of Can dida tropicalis. Concentrations from 0.03 mu g/ml to 8.0 mu g/ml for A mB and from 0.25 mu g/ml to 64.0 mu g/ml for ethophamide or teclosan w ere prepared in enriched yeast nitrogen base agar to establish the min imum inhibitory concentration by the checkerboard technique. The minim um fungicidal concentration was determined by inoculum subculture on S abouraud dextrose agar. In concentrations up to 64.0 mu g/ml, the halo acetamide derivatives did not show antifungal activity against Candida strains. Combinations between ethophamide or teclosan with AmB showed synergistic interactions against most of the studied strains, standin g out the fungicidal activity of the AmB-ethophamide combination again st all C.albicans strains. These results show good perspectives for ex perimental in vivo studies with combined therapy.