OXYGEN-ELECTRODE WITH PYROLYZED COTMPP CATALYST - APPLICATION IN GLUCOSE BIOSENSOR

Amperometric oxygen sensors employing non-platinum electrocatalyst mad e from pyrolyzed cobalt-tetramethoxy-phenylporphyrin (CoTMPP) are desc ribed. A technique for the preparation of an oxygen working electrode with catalyst from a pressed matrix (tablet) is presented. This techni que allows manufacturing numerous electrodes from the same pressed tab let, all of which demonstrate good reproducibility of their parameters . Glucose biosensors are constructed using this oxygen electrode as a basic transducer for amperometric detection of oxygen consumption. The enzyme (glucose oxidase) is held in a micro-bioreactor (face side clo sed by a Silastic coated polycarbonate membrane) in which the oxygen s ensor is immersed. Linearity of the sensor response to glucose concent rations up to 16 mM is reported. Sensors demonstrated a stable signal (varying within +/- 5% of its average value) during 15 days of continu ous in vitro glucose monitoring at 37 degrees C.