AMPEROMETRIC IMMUNOSENSOR FOR THE DETECTION OF 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) IN WATER

An amperometric immunosensor for the determination of the herbicide 2, 4-dichlorophenoxyacetic acid (2,4-D) in water has been developed using sequential injection analysis techniques. The system is based on a ra pid competitive enzyme immunoassay employing an alkaline phosphatase-l abeled monoclonal antibody directed against the herbicide and an immun oreactor with 2,4-D immobilized via bovine serum albumin either to Eup ergit in a column or directly to the surface of a glass capillary. The detection limit of the immunosensor at 0.1 mu g 2,4-D/l without enric hment of the analyte makes automatic measurements of 2,4-D in drinking and ground water feasible.