QUANTITATIVE-DETERMINATION BY USING HPLC AND GLC METHODS FOR COCAINE HCL IN SYNTHETIC BINARY-MIXTURES WITH PROCAINE HCL, LIDOCAINE HCL AND CAFFEINE

Cocaine HCl is a substance which creates psychological and physical de pendence. Usually it is available on the market being diluted by other substances, like local anesthetics, analeptics and CNS stimulating ag ents and inert substance. In this study, HPLC and GLC methods were app lied for qualitative and quantitative determinations of synthetic bina ry mixtures. In the HPLC determination, mu Bondopack C-18 10 mu m. col umn system, a mobile phase consisting of methanol-water-phosphoric aci d-1% hexylamine (75:175:250:3,5) and U.V. detection by photodiodearray (196-500 nm) were used. The linear concentration areas were found in a range of 2.5-25 mu g/mL. The R.S.D percentages for cocaine HCl, proc aine HCl, lidocaine HCl and caffeine were found as 0.922, 0.568, 1.18 and 1.04, respectively. In the GLC determination, two different column systems, a 2% OV-17- Gas Chrom W-HP 100-200 mesh filled column and a 0.25 SE-52 fused silica capillary column, were used. Nitrogen was used in a filled column and helium was used in a capillary column. Mobile phase flow rates were set as 30 mL/min and a flame ionization detector was used with both column systems. The linear concentration intervals were found in a range of 2-25 mu L/mL in both methods. The R.S.D. for cocaine HCl, procaine HCl, lidocaine HCl, and caffeine were found to be 0.907, 0.948, 0.770, 0.901 in the filled column. For cocaine HCl, p rocaine HCl and caffeine R.S.D.'s of 0.774, 0.809, 0.814 were found, w hen a capillary column was used. In quantitative determinations, antip yrine was chosen as internal standard in the HPLC and GLC methods.