A 10-CM YAC CONTIG SPANNING GLC1A, THE PRIMARY OPEN-ANGLE GLAUCOMA LOCUS AT 1Q23-Q25

Primary open-angle glaucoma is a complex of ocular disorders character ized by irreversible lesions of ?he optic nerve, open angle of the ant erior chamber of the eye and elevated intraocular pressures, GLC1A, a locus involved in one form of this disease, has been mapped to an appr oximately 9-cM interval within 1q23-q25, between markers D1S445 and D1 S416/D1S480. A 10-cM yeast artificial chromosome (YAC) contig spanning the whole region is described. This contig is based on 67 YACs, and 4 1 sequence tagged sites comprising 23 generic markers, 16 YAC ends and 2 expressed sequence tags. The reagents reported in this study should be useful tools for the identification of the GLC1A gene by positiona l cloning.