PURE FAMILIAL SPASTIC PARAPLEGIA - CLINICAL AND GENETIC-ANALYSIS OF 9BELGIAN PEDIGREES

We ascertained 9 multigeneration Belgian families with pure dominant s pastic paraplegia (SPG) for clinical and genetic studies, Linkage was examined using simple tandem repeat (STR) market-a located near the 5 loci for familial SPG on chromosomes Xq28 (SPG1), Xq21.3-q22 (SPG2), 2 p21-p24 (SPG4), 14q12-q23 (SPG3) and 15q11.1 (SDG6). Positive linkage results were obtained only for markers at the SPG4 locus mapping the S PG4 gene between D2S400 and D2S367, a region of 4 cM, In order to faci litate the positional cloning of the SPG4 gene, we constructed a conti guous YAC map coveting the SPG4 candidate region, Our physical mapping data Indicate that the SPG4 gene resides within maximal 5 Mb.