Dr. Collingridge et al., MEASUREMENT OF TUMOR OXYGENATION - A COMPARISON BETWEEN POLAROGRAPHICNEEDLE ELECTRODES AND A TIME-RESOLVED LUMINESCENCE-BASED OPTICAL SENSOR, Radiation research, 147(3), 1997, pp. 329-334
A novel oxygen sensor which does not rely on electrochemical reduction
has been used to measure the oxygenation of the murine sarcoma F in a
comparative study with an existing polarographic electrode that is av
ailable commercially. The prototype luminescence sensor yielded an oxy
gen distribution comparable with readings made using a pO(2) histograp
h. The percentage of regions detected that had a pO(2) less than 5 mm
Hg was 79 and 75 using the Eppendorf pO(2) histograph and the luminesc
ence fiber optic sensor, respectively. These values were compatible wi
th a measured radiobiologically hypoxic fraction of 67% in this tumor.
The polarographic method detected more regions with a pO, of 2.5 mm H
g or less (69%) compared with the optical sensor (50%) (P < 0.05). Thi
s could reflect differences in the oxygen use of the sensing devices.
This initial assessment indicates the potential of a fiber-optic-based
oxygen-monitoring system. Such a system should have several advantage
s including monitoring temporal oxygen changes in a given microregion
and use with NMR procedures. (C) 1997 by Radiation Research Society.