P. Campinsfalco et al., DEVELOPMENT OF THE H-POINT STANDARD ADDITIONS METHOD FOR THE USE OF SPECTROFLUOROMETRY AND SYNCHRONOUS SPECTROFLUOROMETRY, Analyst, 119(9), 1994, pp. 2123-2127
The basis of the H-point standard additions method (HPSAM) is develope
d for the use of fluorimetric and synchronous spectrofluorimetric meas
urements. The method permits the transformation of the indeterminate e
rror resulting from the presence of an interference in the determinati
on of an analyte into a systematic constant error that can be evaluate
d and permits the determination of the analyte free from bias error. S
ome examples are presented. Phenol fluorescence calibrations in water
have a high interference from the Raman emission band of the solvent,
The HPSAM permits the elimination of this interference, because the me
thod can eliminate the blank bias error. Resolution of highly overlapp
ed species, such as phenol-o-cresol mixtures, can be carried out with
the method. In order to show this possibility, synchronous spectrofluo
rimetry was chosen to eliminate the blank signal.