IDENTIFICATION OF GENES DIFFERENTIALLY EXPRESSED IN NORMAL LUNG AND NONSMALL CELL LUNG-CARCINOMA TISSUE

Using a magnet-assisted subtraction technique, 17 complementary DNA (c DNA) clones were isolated that were expressed in the normal lung but w ere decreased or lost in the corresponding tumor tissue of a nonsmall cell lung carcinoma patient. The lack of expression of six magnet-assi sted subtraction technique cDNA clones in three additional non-small c ell lung carcinoma cases indicates their possible relevance for non-sm all cell lung carcinoma. Two cDNA clones revealed homology to genes sp ecifically expressed in lung, ie., pulmonary surfactant-associated pro tein B and the receptor for advanced glycosylation end products of pro teins. Three cDNA clones showed identity to cDNA sequences encoding ca lmodulin-like protein, glutamine synthetase, and cytoskeletal beta-act in. One cDNA clone is identical to a recently described human expresse d sequence tag whose gene is still unknown.