DISTRIBUTION AND MICROCHEMICAL DETECTION OF PHENOLIC-ACIDS, FLAVONOIDS, AND PHENOLIC-ACID AMIDES IN MAIZE KERNELS

Authors
SEN A BERGVINSON D MILLER SS ATKINSON J FULCHER RG ARNASON JT
Citation
A. Sen et al., DISTRIBUTION AND MICROCHEMICAL DETECTION OF PHENOLIC-ACIDS, FLAVONOIDS, AND PHENOLIC-ACID AMIDES IN MAIZE KERNELS, Journal of agricultural and food chemistry, 42(9), 1994, pp. 1879-1883
Citations number
26
Categorie Soggetti
Food Science & Tenology",Agriculture,"Chemistry Applied
Journal title
Journal of agricultural and food chemistry → ACNP
ISSN journal
00218561
Volume
42
Issue
9
Year of publication
1994
Pages
1879 - 1883
Database
ISI
SICI code
0021-8561(1994)42:9<1879:DAMDOP>2.0.ZU;2-X
Abstract
Because of the importance of phenolics in the resistance of maize kern els to pests, microspectrofluorometry was employed as a new method of mapping the location of these substances in maize kernels. Autofluores cence due to phenolic acids was detected mainly in the embryo, aleuron e, and pericarp of maize kernel cross sections. Resistant maize types showed higher intensities of phenolic fluorescence but no unusual dist ributions. Borinic acid reagent enhanced fluorescence due to flavonoid s in the aleurone layer, and Ehrlich's reagent revealed phenolic acid amide fluorescence in the embryo and aleurone. The localization of phe nolic amines was confirmed by HPLC analysis.