We report the identification of a family of sequences located by in si
tu hybridisation to the centromeres of all the Triticeae chromosomes s
tudied, including the supernumerary and midget chromosomes, the centro
meres of all maize chromosomes and the heterochromatic regions of rice
chromosomes. This family of sequences (CCS1), together with the cerea
l genome alignments, will allow the evolution of the cereal centromere
s and their sites to be studied. The family of sequences also shows ho
mology to the CENP-B box. The centromeres of the cereal species and th
e proteins that interact with them can now be characterised.