THE SEC-1 LOCUS ON THE SHORT ARM OF CHROMOSOME 1R OF RYE (SECALE-CEREALE)

This paper describes a detailed sequence analysis of the omega-secalin gene array at the Sec-1 locus on the short arm of chromosome 1 of rye . The analysis shows that the genes are separated by 8 kb of spacer se quence and that the gene/spacer units are arranged in a head to tail f ashion. The boundaries of the array are identified, and a fragment con taining the majority of the genes in the array is separated by PFG ana lysis. The sequence data of one 9.2 kb gene unit have been determined, and because of the similarity of the gene units within the array thes e data provide a detailed sequence analysis of 140 kb of the Sec-1 loc us. Fluorescence in situ hybridization, using lambda clones isolated f or the structural analysis, identifies the position of the array on th e rye chromosomes relative to the 5S rRNA genes.