STUDY OF CELL-DYN-3500 IN EXTREME VALUES

The quality tests made during the preparation of labile blood products permit to validate the product and to make sure the making procedures are applied properly. The labile blood products have to correspond to a number of standards set by a ministerial order. It is thus necessar y to check the manufacturing unit of some products. On account of the original technology of Cell-Dyn 3500 which associates methods of imped ance variation mesure analysis and flow cytometric technique analysis on non-marked cells, and of its veterinary modulus permitting counting in extreme values, it seemed to be worth being evaluated in a blood b ank quality control laboratory. Linearity was studied from a range of dilutions obtained from high value samples prepared by the centrifugat ion of blood bags and collection of the buffy coat by Optipress system . The study was performed on 22 samples. The linearity in the counting of red cells, white cells, platelets and haematocrit was mesured. Int ersample contamination was mesured. It focused on the values of platel ets and white cells. A sample of high cell concentration prepared in t he condition mentionned above and a white cell-reduced blood concentra te were used. The study of repeatability was done by treating 20 times three samples covering a wide range of values of the different parame ters of cell numeration. The linearity concerning parameters and the r ange of the values studied are good (example: from 10 to 3000 10(9)/l for platelets). Contamination is low for white cells and non appreciab le for platelets. Repeatability shows excellent variation coefficients .