RELATION BETWEEN PLATINUM-DNA ADDUCTS AND COMPLETE REMISSION IN ADULTACUTE NONLYMPHOCYTIC LEUKEMIA

The purpose of this study was to determine the relationship between pl atinum-DNA adducts in leukemic cells and the success of remission indu ction therapy in adult patients with acute nonlymphocytic leukemia (AN LL). Freshly isolated cells from pre-treatment bone marrow aspirates o f 14 patients were incubated with cisplatin in vitro and the amount of platinum bound to DNA was determined using a competitive ELISA proced ure and an antibody directed against platinum-modified DNA. Platinum-D NA adduct levels discriminated well between complete remission (CR) an d remission failure (RF) patients, treated with mitoxantrone, cytosine arabinoside +/- carboplatin.