COMPARISON OF EFFECTS OF ANGIOTENSIN-CONVERTING ENZYME-INHIBITION WITH THOSE OF ANGIOTENSIN-II RECEPTOR ANTAGONISM ON FUNCTIONAL AND METABOLIC RECOVERY IN POSTISCHEMIC WORKING RAT-HEART AS STUDIED BY [P-31] NUCLEAR-MAGNETIC-RESONANCE
Jg. Werrmann et Sm. Cohen, COMPARISON OF EFFECTS OF ANGIOTENSIN-CONVERTING ENZYME-INHIBITION WITH THOSE OF ANGIOTENSIN-II RECEPTOR ANTAGONISM ON FUNCTIONAL AND METABOLIC RECOVERY IN POSTISCHEMIC WORKING RAT-HEART AS STUDIED BY [P-31] NUCLEAR-MAGNETIC-RESONANCE, Journal of cardiovascular pharmacology, 24(4), 1994, pp. 573-586
To assess the role of angiotensin II (AII) in development of myocardia
l injury during ischemia and reperfusion, the effects of short-term tr
eatment with the angiotensin-converting enzyme (ACE) inhibitor lisinop
ril were compared with the effects of short-term treatment with L-158,
338, an AII antagonist, in isolated working rat heart. Myocardial func
tion was assessed and correlated with simultaneous measurement of high
-energy phosphate metabolism and intracellular pH by [P-31] nuclear ma
gnetic resonance (NMR) before, during, and after global ischemia. Hear
ts from rats treated with 1 mg/kg lisinopril in vivo recovered substan
tially more function than those of controls (p < 0.001), whereas 50 ng
/ml (0.11 mu M) lisinopril in vitro had no effect on functional recove
ry. A dose-dependent increase in functional recovery was observed in r
at heart treated with 0.3, 1, or 3 mg/kg L-158,338 in vivo (p < 0.005)
. Treatment with 50 ng/ml (0.12 mu M) L-158,338 in vitro also resulted
in increased functional recovery (p < 0.02). Significantly milder aci
dosis during ischemia and significantly increased coronary flow were c
haracteristic of the improved functional recovery exhibited by the gro
ups treated with either lisinopril or L-158,338 in vivo. Treatment wit
h L-158,338 in vitro caused significantly increased coronary flow duri
ng reperfusion as compared with either its control group or with lisin
opril treatment in vitro. High-energy phosphate metabolism was essenti
ally unchanged by any treatment regimen. AII antagonism alone resulted
in a degree of improvement in functional recovery comparable to that
observed with oral ACE inhibitor treatment.