INFUSION OF LARGE QUANTITIES OF AUTOLOGOUS BLOOD MONOCYTE-DERIVED MACROPHAGES IN 2 CANCER-PATIENTS DID NOT INDUCE INCREASED CONCENTRATION OF IL-6, TNF-ALPHA, SOLUBLE CD14 AND NITRATE IN BLOOD-PLASMA

In an attempt to increase tile number of macrophages available for rei nfusion in immunotherapy trials, GM-CSF was injected in vivo to mobili ze circulating blood-monocytes in 2 cancer patients. Subsequently mono nuclear cells were collected by apheresis, cultured in the presence of GM-CSF and activated with IFN-gamma. This procedure resulted in the h al vesting of 1.3 to 3.1 x 10(9) (mean 2 x 10(9)) macrophages per aphe resis, product which was very well tolerated at autologous reinfusion. These infusions did not induce increased levels of TNF-alpha, IL-6, s oluble CD14 nor nitrates in blood plasma (or urine). The lack of TNF-a lpha and IL-6 release in blood plasma could explain the good tolerance of these infusions. No invivo anti-tumoural activity of these high nu mbers of infused macrophages could be observed.