EFFECTS OF U46619 AND OF INHIBITORS OF THE SYNTHESIS OF TXA(2) ON INSULIN ACTION ON THE METABOLISM OF LABELED GLUCOSE IN UTERI ISOLATED FROM OVARIECTOMIZED-DIABETIC RATS

The production of (CO2)-C-14 from labelled glucose by isolated uterine strips from ovariectomized-diabetic rats has been studied. U46619, an analogue of TXA(2) did not affect basal glucose metabolism; however, insulin-induced increment in CO2 production was completely blocked, bo th in ovariectomized (OVD) or ovariectomized-estrogenized (OVED) diabe tic uterus. OKY064 as well as UK38485, both inhibitors of TXA(2) synth esis, stimulated glucose metabolism (p<0.05) similar to that of insuli n in uterine tissue from OVD and OVED rats. Inhibition in the synthesi s and release of TXB(2) was detected (p<0.01) by uterine radioconversi on of C-14-arachidonic acid when adding OKY38485 to the incubation med ium, and the production of other prostanoids such as 6-keto-PGF(1 alph a), PGF(2 alpha) and PGE(2) was enhanced. In summary, TXA(2) inhibited insulin-induced glucose metabolism in diabetic animals.