Sn. Coelho et al., IMMUNOLOGICAL DETERMINANTS OF SUSCEPTIBILITY TO EXPERIMENTAL GLOMERULONEPHRITIS - ROLE OF CELLULAR-IMMUNITY, Kidney international, 51(3), 1997, pp. 646-652
To identify the immunologic mechanisms that influence susceptibility t
o GN, we compared the severity of accelerated anti-glomerular basement
membrane (GBM) nephritis between Lewis (LEW) and Brown Norway (BN) ra
ts and analyzed differences in their immune responses to the nephritog
enic immunoglobulin. Lewis (LEW) rats preimmunized with sheep IgG deve
loped proliferative GN with marked proteinuria [peak protein excretion
(mean +/- SEM) = 85.3 +/- 15.3 mg/24 hr; normal = 6.4 +/- 0.8 mg/24 h
r] after receiving a subnephritogenic dose of sheep anti-rat GBM antis
erum. Identically treated Brown Norway (BN) rats, on the other hand, h
ad minimal renal pathology and minimal proteinuria (peak protein excre
tion = 22.6 +/- 3.1 mg/24 hr; normal = 13.0 +/- 0.6 mg/24 hr). Serum t
iters of rat anti-sheep IgG isotypes and intraglomerular binding of sh
eep IgG, rat IgG, and rat complement (C-3) were comparable in both str
ains. In contrast, only LEW rats developed a strong cellular immune re
sponse to sharp IgG represented by intrarenal T lymphocyte (OX19(+)) a
nd monocyte (ED1(+)) accumulation [LEW vs. BN (mean +/- SEM): OX19(+)
= 0.60 +/- 0.10 vs. 0.14 +/- 0.01 cells/glomerulus, control = 0.02 +/-
0.01; ED1(+) = 4.0 +/- 0.4 vs. 1.0 +/- 0.2 cells/glom., control = 0.8
+/- 0.3] and a significant cutaneous delayed-type hypersensitivity (D
TH) reaction [LEW versus BN (mean I SEM): Delta ear thickness = 0.22 /- 0.02 vs. 0.05 +/- 0.03 mm; control = 0.04 +/- 0.02 mm]. Upon rechal
lenge with sheep IgG in vitro, LEW splenocytes expressed a T helper 1
(Th1) cytokine pattern (IFN gamma and IL-2 mRNA, but little IL-4 mRNA)
which is associated with delayed-type hypersensitivity reactions. BN
splenocytes, on the other hand, expressed IL-4 in addition to IL-2 and
IFN gamma mRNA that is consistent with an undifferentiated (Th0) cyto
kine profile. These studies suggest that humoral immunity to heterolog
ous immunoglobulin planted In the kidney is not sufficient for full ex
pression of accelerated anti-GBM nephritis, and that additional cellul
ar immune mechanisms are required. We conclude that susceptibility to
accelerated anti-GEM nephritis is strongly influenced by the host's pr
opensity to mount a Th1-type response and DTH reaction to the disease-
inciting immunoglobulin.