AN APPROACH FOR RAPID CHECKING OF SEED PURITY BY RFLP ANALYSIS OF NUCLEAR-DNA IN F1-HYBRID OF CUCUMBER (CUCUMIS-SATIVUS L)

In F1 hybrid seed production, the elimination of contaminated self- an d/or sib-crossed seeds from hybrid seeds is an important subject. We a ttempted to use RFLP analysis of nuclear DNA for checking the seed pur ity of the F1 hybrid cucumber. Between the two parental lines which ar e promising for an F1 cucumber cultivar, two RFLP clones were establis hed. The frequency of RFLP clones in the genomic library was 1%. Two m icrograms of DNA, which could be isolated from a 9-day-old seedling, w ere sufficient for RFLP detection with non-radioactive Southern hybrid ization. This system enabled a practical and a rapid check of the seed purity in an F1 hybrid cucumber.