EFFECTS OF NAPHTHALENEACETIC ACID AND BEN ZYLADENINE ON GROWTH OF BULBLETS REGENERATED FROM WHITE CALLUS OF MOTHER SCALE OF LILIUM-JAPONICUM THUNB

White callus induced from mother scale of Lillium japonicum Thunb. was cultured on MS-medium without plant hormones under continuous 2000 lx at 25-degrees-C in vitro. A bulblet regenerated on the white callus w as used for treatments.1. Small bulblets, 15 to 18 mg (FW), were imbed ded on MS-media containing of NAA (0.2, 1.0 and 5.0ppm) and BA (0.2, 1 .0 and 5.0ppm), and then cultured for 8 weeks. The grown bulblets coul d be classified into three types; normal type, abnormal type-I and abn ormal type-II. The morphology of normal bulblets was comparable to bul blets that formed on mother scales cultured in vitro. The bulblets of abnormal type-I were globular and the scale grew more slowly than that of normal ones. Scale of the type-II became dropsy and grew very slow ly. 2. The regenerated bulblets which were cultured on a medium of 0.1 ppm NAA and 0.01 ppm BA gained the most fresh weight and had the most and longest roots per bulblet compared to the other treatments. Leaf emergence was advanced by the medium of 0.01 ppm NAA and 0.1 ppm BA. H istologically, root tips of bulblets grown on the medium of 1.0 ppm NA A and no BA for 8 weeks had two central cylinders, and a large hollow core in the cortex. Cracks in the epidermis also developed on this med ium. 3. When the bulblets with scale leaves were cultured on the mediu m of 0.1 ppm NAA and no BA for 4 weeks, they has grown into a plantlet with roots. We conclude that the combination of 0.1 to 0.01 of NAA an d BA, respectively, were optimum for the growth and rooting of bulblet s.