PHOTOCHEMICAL CONVERSION OF OLIGONUCLEOTIDES CONTAINING 2-CHLORO-2'-DEOXYADENOSINE - ENHANCED UV SENSITIVITY OF A MODIFIED PURINE BASE-INDUCED BY THE NEAREST-NEIGHBOR

Authors
RAMZAEVA N SEELA F
Citation
N. Ramzaeva et F. Seela, PHOTOCHEMICAL CONVERSION OF OLIGONUCLEOTIDES CONTAINING 2-CHLORO-2'-DEOXYADENOSINE - ENHANCED UV SENSITIVITY OF A MODIFIED PURINE BASE-INDUCED BY THE NEAREST-NEIGHBOR, Chemical research in toxicology, 7(5), 1994, pp. 643-649
Citations number
33
Categorie Soggetti
Toxicology,Chemistry
Journal title
Chemical research in toxicology → ACNP
ISSN journal
0893228X
Volume
7
Issue
5
Year of publication
1994
Pages
643 - 649
Database
ISI
SICI code
0893-228X(1994)7:5<643:PCOOC2>2.0.ZU;2-X
Abstract
Irradiation of alternating dodecamers containing 2-chloro-2'-deoxyaden osine (Cl(2)dAdo) with ultraviolet light (254 nm) has been investigate d. The photoconversion of Cl(2)dAdo into 2'-deoxyisoguanosine (isodGuo ) and the completeness of the reaction were studied by reversed-phase HPLC and UV absorption spectra. The photosensitivity of Cl(2)dAdo with in an oligonucleotide was found to be sequence-specific and depends on the nearest neighbor; 2'-deoxyguanosine residues accelerate the rate of photoconversion by a factor of 3.