Tv. Kulakovskaya et al., ATPASE OF THE PLASMA-MEMBRANE OP THE YEAST YARROWIA-LIPOLYTICA IS NOTINVOLVED IN CITRATE EXCRETION, Microbiology, 63(1), 1994, pp. 12-15
The activity of the plasma membrane ATPase of Yarrowia lipolytica decr
eased tenfold during the course of nitrogen-limited growth in medium w
ith glucose; the capability of cells for glucose-dependent medium acid
ification also decreased. Stationary-phase cells were capable of citra
te excretion; within the first 30 min this process was glucose-indepen
dent and resistant to diethylstilbestrol (inhibitor of the plasmalemma
ATPase). Citric acid excretion by Y. lipolytica is assumed to be ener
gy-independent.