IDENTIFICATION OF RANDOM CULTIVAR LINES OF TOMATO (LYCOPERSICON-ESCULENTUM) FROM GENE MARKERS DEVELOPED USING CELLULOSE-ACETATE ELECTROPHORESIS

Citation
Pb. Mather et al., IDENTIFICATION OF RANDOM CULTIVAR LINES OF TOMATO (LYCOPERSICON-ESCULENTUM) FROM GENE MARKERS DEVELOPED USING CELLULOSE-ACETATE ELECTROPHORESIS, Seed science and technology, 21(3), 1993, pp. 643-651
Citations number
NO
Categorie Soggetti
Agriculture,"Plant Sciences",Horticulture
Journal title
ISSN journal
02510952
Volume
21
Issue
3
Year of publication
1993
Pages
643 - 651
Database
ISI
SICI code
0251-0952(1993)21:3<643:IORCLO>2.0.ZU;2-Z
Abstract
Cellulose-acetate electrophoresis of individual tomato seeds was used to examine the potential of allozymes as gene markers to differentiate between a random selection of tomato cultivars. This study demonstrat ed that a large amount of allelic variation was present in forty-four randomly selected tomato cultivars and sufficient diversity existed to differentiate 92% of pairwise comparisons. This combined with the abi lity of individual seeds to provide enough sample for typing at up to 10 enzyme loci demonstrates that cellulose-acetate electrophoresis is an ideal tool for use in genetic improvement programs on tomato cultiv ars.