M. Okada et al., HISTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY OF EXTRACELLULAR-MATRIX OF RAT FETAL DUCTUS-ARTERIOSUS DURING THE INDOMETHACIN-INDUCED CONSTRICTION, Jikken Dobutsu, 43(4), 1994, pp. 551-558
Administration of anti-inflammatory non-steroidal drugs to pregnant an
imals causes constriction of the fetal ductus arteriosus (DA). To clar
ify the mechanism of the indomethacin-induced constriction of the rat
fetal DA, extracellular matrix (ECM) components were observed by immun
ohistochemical techniques with antibodies 2B1, 6B6, 3B3, 9A2 and other
s to various kinds of proteoglycans (PGs). In the 10mg/kg group, the l
arge PG revealed with antibody 2B1 was increased in the inner and oute
r media at constriction, and chondroitin 6-sulfate PG was strongly imm
unostained with antibody 3B3 as granular substances in the outer media
, although no difference in stainability of chondroitin 4-sulfate PG r
evealed with the antibody 9A2 was observed between experimental and co
ntrol groups. This suggests that the cells composing the fetal DA show
ed a tendency of proliferation and resultant production and aggregatio
n of large PG with chondroitin 6-sulfate chains during constriction in
duced by indomethacin-treatment.