DETERMINATION OF 1-HYDROXYPYRENE IN RAT URINE USING LIQUID EXTRACTIONAND HPLC UV-DETECTION METHOD

Some modifications of HPLC method for identification 1-hydroxypyrene i n rat urine are described. As opposed to analytical procedure presente d in literature which includes solid phase extraction (SPE) and HPLC d etermination with fluorescence detection, more approachable extraction with cyclohexane and detection in the UV range were used after enzymi c hydrolysis. 1-Hydroxypyrene could be detected in the urine of rats w ith 88.3 % average recovery level and 0.25 mug l-1 urine detection lim it. After pretreatment rats with pyrene p.o. in dose 20 mg kg-1 excret ion of 1-hydroxyprene during 72 h increased in urine from 0.22 to 105. 7 mug per mg of creatinine.