IMMUNE-RESPONSES TO SUPEROXIDE-DISMUTASE AND SYNTHETIC PEPTIDES OF SUPEROXIDE-DISMUTASE IN CATTLE VACCINATED WITH BRUCELLA-ABORTUS STRAIN-19 OR STRAIN-RB51

Antibody and lymph node cell-mediated immune responses to recombinant Brucella abortus strain 19 Cu-Zn superoxide dismutase (rSOD) and to th ree synthetic strain 19 Cu-Zn SOD peptides were measured during 2 to 1 2 weeks following vaccination of cattle with B. abortus strain 19 or R B51. Cattle vaccinated with strain 19 or RB51 did not produce antibody to rSOD and to the SOD peptides. Lymph node cells from cattle vaccina ted with strain 19, but not with strain RB51, proliferated when incuba ted with either rSOD or one of the three tested SOD peptides (GGDNYSDK PEPLGG). These results suggest that neither the strain 19 nor the stra in RB51 vaccine induces antibody production to SOD and only the strain 19 vaccine induces lymph node cell-mediated immune responses to SOD.