In a process called ''molecular combing,'' DNA molecules attached at o
ne end to a solid surface were extended and aligned by a receding air-
water interface and left to dry on the surface. Molecular combing was
observed to extend the length of the bacteriophage lambda DNA molecule
to 21.5 +/- 0.5 micrometers (unextended length, 16.2 micrometers). Wi
th the combing process, it was possible to (i) extend a chromosomal Es
cherichia coli DNA fragment (10(6) base pairs) and (ii) detect a minut
e quantity of DNA (10(3) molecules). These results open the way for a
faster physical mapping of the genome and for the detection of small q
uantities of target DNA from a population of molecules.