TRANSCRIPTIONAL REGULATION OF THE TRICHODERMA-LONGIBRACHIATUM EGL1 GENE

Transcription of the Trichoderma longibrachiatum egl1 gene is induced in the presence of lactose and beta-methylglucoside and repressed by g lucose. A DNA fragment containing 722 bp upstream of the ATG codon has been sequenced. The gene has two major transcription start points (20 and 24 nucleotides upstream from the ATG codon) and several transcrip tion termination points (located in a region around 130 nt downstream of the stop codon). Two 6-mer sequences (5'-CTGGAG-3') separated by 16 bp are present in the egl1 gene promoter. These sequences match the A spergillus nidulans consensus CreA binding site and might be implicate d in carbon catabolite repression of egl1 transcription.