Water-holding properties have been well recognized by food technologis
ts among the diversity of functional properties attributed to milk pro
tein products. In general, water-holding is accomplished by a complexi
ty of interactions between water and milk proteins. Besides the term w
ater-holding, synonyms such as water retention, imbibing and hydration
have been used to describe this phenomenon. This paper provides a cle
arer understanding of this parameter by considering some fundamentals
of both the molecular structure of milk proteins and the physical inte
rrelationships between water and milk protein powder particles. Differ
ences in water-holding properties of milk protein products are frequen
tly observed and may be due to the nature of the protein and to techno
logical influences. Methods measuring water absorption and methods tha
t measure water retention are applied for the examination of water-hol
ding. By following this distinction, the principles of various methods
(e.g., the Baumann method, the absorption capacity test, Farinographi
c procedures, net tests, filtration tests, modern instrumental techniq
ues) are reviewed.