L. Eynard et al., DETERMINATION OF WHEAT PROTEINS IN SOLUTION BY DYE BINDING IN FLOUR, DOUGH, AND BREAD CRUMB, Cereal chemistry, 71(5), 1994, pp. 434-438
Color development by the Bradford reagent with soluble wheat proteins
has been examined so as to allow an easy and reliable application. The
range examined was 0.3-3.5 mg/ml of protein. Second-order regressions
fully interpret the nonlinearity in the color response. The reasons f
or nonlinearity were investigated. Commercial gliadin, standard gluten
, water and an acid extract of wheat flour, and bovine serum albumin w
ere assayed. The absorbance developed per unit weight differed. The sp
ectral response depended on the polypeptide composition of the samples
assayed. Dye binding proved to be a rapid and reliable technique for
the routine assay of soluble proteins in wheat flour, in dough, and af
ter baking. Data obtained is in full accordance with total nitrogen de
terminations.