THE BACTERIOCHLOROPHYLL BIOSYNTHESIS GENE, BCHM, OF RHODOBACTER-SPHAEROIDES ENCODES S-ADENOSYL-L-METHIONINE - MG PROTOPORPHYRIN-IX METHYLTRANSFERASE

Authors
GIBSON LCD HUNTER CN
Citation
Lcd. Gibson et Cn. Hunter, THE BACTERIOCHLOROPHYLL BIOSYNTHESIS GENE, BCHM, OF RHODOBACTER-SPHAEROIDES ENCODES S-ADENOSYL-L-METHIONINE - MG PROTOPORPHYRIN-IX METHYLTRANSFERASE, FEBS letters, 352(2), 1994, pp. 127-130
Citations number
22
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
352
Issue
2
Year of publication
1994
Pages
127 - 130
Database
ISI
SICI code
0014-5793(1994)352:2<127:TBBGBO>2.0.ZU;2-T
Abstract
The bchM gene of Rhodobacter sphaeroides has been sequenced and then o verexpressed in E. coli producing a protein of M(r) approximately 27,5 00. Cell-free extracts of the transformed E. coli strain are able to m ethylate added Mg protoporphyrin, resulting in the formation of Mg pro toporphyrin monomethyl ester. The identity of this product was verifie d by HPLC. The bchM gene product is therefore assigned to the methyltr ansferase step in bacteriochlorophyll biosynthesis.