MOLECULAR-CLONING OF BAC7, A PROLINE-RICH AND ARGININE-RICH ANTIMICROBIAL PEPTIDE FROM BOVINE NEUTROPHILS

Authors
SCOCCHI M ROMEO D ZANETTI M
Citation
M. Scocchi et al., MOLECULAR-CLONING OF BAC7, A PROLINE-RICH AND ARGININE-RICH ANTIMICROBIAL PEPTIDE FROM BOVINE NEUTROPHILS, FEBS letters, 352(2), 1994, pp. 197-200
Citations number
34
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
352
Issue
2
Year of publication
1994
Pages
197 - 200
Database
ISI
SICI code
0014-5793(1994)352:2<197:MOBAPA>2.0.ZU;2-M
Abstract
Bac7 is a 7 kDa proline- and arginine-rich antimicrobial peptide which was purified from bovine neutrophils. We have used PCR to clone the c DNA of Bac7 precursor, a polypeptide of 21,569 Da. This cDNA is highly conserved in the 5' region, with respect to the corresponding region in the precursors of several other structurally unrelated myeloid anti microbial peptides. Furthermore, a 148 nt non-coding region at the 3' end is 75% homologous to a corresponding region of the cDNA of the pre cursor of PR-39, a porcine antibacterial peptide which is also proline - and arginine-rich.