A. Tunstall et al., COMPARISON OF TISSUE FACTOR AND PROSTACYCLIN PRODUCTION BY HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS ON DACRON VASCULAR PROTHESES AND DACRON SMOOTH FILMS, Journal of biomedical materials research, 28(10), 1994, pp. 1233-1238
functional capacity of human umbilical vein endothelial cells (HUVEC)
grown on Dacron (polyethylene terephthalate; PET) vascular prosthetic
material was compared with the function of cells on smooth surfaced PE
T, tissue culture polystyrene (TCPS), and Natrix-coated TCPS. Prosthet
ic materials include two knitted fabrics (Bionit I and II) and two wov
en preparations (DeBakey Soft Woven and Extra Low Porosity). Two entit
ies produced by HUVEC that influence blood coagulation were assessed:
the procoagulant tissue factor (TF) and the anticoagulant prostacyclin
(PGI(2)). Although TF activity was stimulated on all substrates by en
dotoxin (LPS), there was no difference among prostheses and no differe
nce among smooth surface materials, but TF was reduced in cells on the
prosthetic materials relative to those on smooth surface substrates.
The reduced TF production by HUVEC on prosthetic material could be rev
ersed by returning them to TCPS. In contrast, PGI(2) production on pro
stheses was comparable to that on smooth surfaces for both stimulated
and unstimulated cells. Stimulation with histamine (1 mu M) gave a 2.4
-fold increase in PGI(2) whereas mellitin (10 mu g/ml) increased produ
ction 12.5-fold. The differential response of HUVEC with regard to the
se two coagulation factors, one of which is secreted and the other mem
brane bound, may reflect the distorted shape of cells on fibers of the
prosthesis. (C) 1994 John Wiley & Sons, Inc.