COMPARISON OF TISSUE FACTOR AND PROSTACYCLIN PRODUCTION BY HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS ON DACRON VASCULAR PROTHESES AND DACRON SMOOTH FILMS

Citation
A. Tunstall et al., COMPARISON OF TISSUE FACTOR AND PROSTACYCLIN PRODUCTION BY HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS ON DACRON VASCULAR PROTHESES AND DACRON SMOOTH FILMS, Journal of biomedical materials research, 28(10), 1994, pp. 1233-1238
Citations number
22
Categorie Soggetti
Engineering, Biomedical","Materials Science, Biomaterials
ISSN journal
00219304
Volume
28
Issue
10
Year of publication
1994
Pages
1233 - 1238
Database
ISI
SICI code
0021-9304(1994)28:10<1233:COTFAP>2.0.ZU;2-X
Abstract
functional capacity of human umbilical vein endothelial cells (HUVEC) grown on Dacron (polyethylene terephthalate; PET) vascular prosthetic material was compared with the function of cells on smooth surfaced PE T, tissue culture polystyrene (TCPS), and Natrix-coated TCPS. Prosthet ic materials include two knitted fabrics (Bionit I and II) and two wov en preparations (DeBakey Soft Woven and Extra Low Porosity). Two entit ies produced by HUVEC that influence blood coagulation were assessed: the procoagulant tissue factor (TF) and the anticoagulant prostacyclin (PGI(2)). Although TF activity was stimulated on all substrates by en dotoxin (LPS), there was no difference among prostheses and no differe nce among smooth surface materials, but TF was reduced in cells on the prosthetic materials relative to those on smooth surface substrates. The reduced TF production by HUVEC on prosthetic material could be rev ersed by returning them to TCPS. In contrast, PGI(2) production on pro stheses was comparable to that on smooth surfaces for both stimulated and unstimulated cells. Stimulation with histamine (1 mu M) gave a 2.4 -fold increase in PGI(2) whereas mellitin (10 mu g/ml) increased produ ction 12.5-fold. The differential response of HUVEC with regard to the se two coagulation factors, one of which is secreted and the other mem brane bound, may reflect the distorted shape of cells on fibers of the prosthesis. (C) 1994 John Wiley & Sons, Inc.