ASSIGNMENT OF THE TRUE AND PROCESSED GENES FOR HUMAN GLYCINE DECARBOXYLASE TO 9P23-24 AND 4Q12

Southern analysis using a human glycine decarboxylase cDNA probe and g enomic DNA preparations from Chinese hamster human hybridoma cell line s demonstrated that on the segregated human chromosomes, 4 and 9, ther e are glycine decarboxylase cDNA-related sequences. The finding is con firmatory of the fact that fragments of both the true and processed ge nes for this protein have been cloned. Since one of four HindIII fragm ents revealed in the present Southern analysis matched requirements fo r the property expected from the restriction map reported for the proc essed gene, this was located on chromosome 4. The three remaining sign als were consequently ascribed to fragments from the true gene on chro mosome 9. Fluorescence in situ hybridization using the genomic clones assigned the true and processed genes to 9p23-24 and 4q12, respectivel y. (C) 1994 Academic Press, Inc.