Cf. Cesarone et al., HEPATIC POLY(ADP-RIBOSE) POLYMERASE-ACTIVITY IN RAT IS CONTROLLED BY THYROID-HORMONES, Biochemical and biophysical research communications, 203(3), 1994, pp. 1548-1553
The in vivo effect of the thyroidal state on poly(ADP-ribose) polymera
se activity was investigated in eu- and hypothyroid rats after treatme
nt with L-triiodothyronine. Untreated hypothyroids showed an increased
basal rate of the enzyme. The treatment of both eu- and hypothyroid r
ats with L-triiodothyronine induced a prompt drop of the endogenous ac
tivity not due to a reduction of the catalytic protein. This decrease
well evident 1 h after treatment was transient, returning to controls
values within 8 h. In isolated liver nuclei from euthyroids the in vit
ro exposure to increasing L-triiodothyronine concentrations from 10(-1
8) to 10(-6) M resulted in a progressive inhibition of the enzyme. Thi
s loss in activity was not derived from a reduction of the total level
of the catalytic protein. The pretreatment with the antagonist amioda
rone suppressed the hormone effect,suggesting that nuclear receptors c
ould mediate poly(ADP-ribose) polymerase activity. (C) 1994 Academic P
ress, Inc.